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. 2021 Feb 2;12(1):99–117. doi: 10.1016/j.jcmgh.2021.01.020

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Single-cell expression atlas and cell typing in biopsies of Chinese UC patients and control samples. (A) Experimental design. Fresh biopsy specimens were disassociated and single-cell suspensions were obtained from 4 samples from inflamed sigmoid colon (UC) with the noninflamed ascending colon biopsy specimens (SC), and 4 healthy samples (HCs). (B) The UMAP (Uniform Manifold Approximation and Projection) plot identifies 10 epithelial cell clusters and 11 immune cells from 12 colon biopsies. (C) Violin plots showing the expression distribution of selected marker genes across cell clusters. (D) UMAP shows the lineage markers, PTPRC for immune cells, EPCAM for epithelial cells, COL1A1 for fibroblasts, and VWF for endothelial cells. (E, F) Boxplots showing percentage of epithelial cell (E) and immune cell (F) clusters of total specimens (per biopsy) in inflamed samples relative to noninflamed samples and healthy samples (significant changes of Dirichlet-multinomial regression adjusted with the Benjamini-Hochberg method were marked out). ∗P < .05, ∗∗P < .01.