Fig. 2. IκBζ regulates IL-17A/TNFα-mediated synergistic induction of IL-19 and IL-20. Human keratinocytes were transfected with IκBζ siRNA (siIκBζ) or control siRNA (siCon) before stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml) or the combinations as indicated for 24 hours (n=4). (A, B) mRNA expression was analysed by qPCR, and RPLP0 was used as a reference gene for normalisation. (C, D) Protein level was analysed by ELISA (n=4). Horizontal lines represent medians. IL: interleukin, TNFα: tumour necrosis factor alpha, IκBζ: I-kappa-B-zeta. ^*p<0.05 comparing the cells transfected with IκBζ siRNA (siIκBζ) with the cells transfected with control siRNA (siCon).