Fig. 4. IL-17A and TNFα increased the DNA binding activity of NF-κB. Human keratinocytes were stimulated with IL-17A (100 ng/ml) or TNFα (10 ng/ml) or their combination for one hour before the NF-κB DNA binding activity was analysed by electrophoretic mobility shift assay. Antibodies against p50 and p65 were tested for their ability to cause a super shift of the nuclear complex associated with the probe. A representative result of three separate experiments is shown. IL: interleukin, TNFα: tumour necrosis factor alpha.