Combined effects of orelabrutinib and rituximab in vivo
(A and B) Apoptosis of tumor tissues from TMD8 tumor model was assessed by the terminal deoxynucleotidyl transferase nick-end-labeling (TUNEL) assay, and the nuclei were counterstained with DAPI. Representative images show apoptotic (fragmented) DNA (green staining) and the corresponding cell nuclei (blue) staining. Scale bar: 50 μm. Results are expressed as mean ± SD (n = 3 biologically independent samples). (C and D) Ki67, granzyme B, and NKp44 were assessed by immunohistochemistry of TMD8 tumor tissue. Scale bar: 60 μm. The data show the density of positivity cells for each section. Student’s t test was performed for statistical analysis. Values present as percentages of control group in mean ± SD (n = 3 biologically independent samples), ∗∗p < 0.01 compared with control group, and ∗∗∗p < 0.001 compared with control group; ##p < 0.01 compared with rituximab group and ###p < 0.001 compared with rituximab group.