Figure 2.

The enzyme activity of SIRT1 is negatively correlated with the protein level of TET2. (A,B) SH-SY5Y cells were treated with MPP⁺ (2.5 mM), RV (resveratrol 25 μM) separately or were treated with RV 12 h before MPP⁺ and then co-treated with MPP⁺ for 24 h. Western blot was used to detect the protein level of TET2. (C) qRT-PCR assay was used to test the mRNA level of TET2 in SH-SY5Y cells after SRT1720 (5 μM) or EX-527 (10 μM) were treated for 24 h. (D–G) SH-SY5Y cells were treated with a different dose of SRT1720 or EX-527 for 24 h to examine the protein level of TET2. (H,I) SH-SY5Y cells were transfected with shTET2 or shNC for 48 h. Western blot was used to detect the protein level of TET2 *P < 0.05, **P < 0.01, ***P < 0.001.