Figure 1.

Rotarod training at 5 or 10 weeks of age induces anxiety in adult mice

(A) Outline of our experimental design.

(B) Arrangement of behavioral paradigms for (C and D). A rotarod test (RR) was carried out on mice at 5 weeks (w) of age, followed by open field (OF) and elevated plus maze (EPM) as indicated.

(C) The results of open field test on mice with rotarod training at 5 weeks of age.

(D) The results of elevated plus maze test of mice with rotarod training at 5 weeks of age (n = 9 for WT–RR, n = 8 for WT + RR).

(E) Arrangement of behavioral paradigms for (F)-(G). Mice were subjected to rotarod training at 10 weeks of age.

(F) The results of open field test on mice with rotarod training at 10 weeks of age.

(G) The results of elevated plus maze test on mice with rotarod training at 10 weeks of age (n = 8 for WT–RR mice, n = 9 for WT + RR mice).

Data represent mean plus SEM. Two-tailed Mann-Whitney U test (B-C). ∗p < 0.05; ∗∗∗p < 0.001.