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. 2021 Apr 15;12:645723. doi: 10.3389/fphys.2021.645723

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Copyright © 2021 Stocco, Sfriso, Borile, Contran, Barbon, Romanato, Macchi, Guidolin, De Caro and Porzionato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Evidence for the existence of A_2AR–D₂R heterodimers in rat CB samples by PLA. Red dots showed the proximity of adenosine A_2AR and dopamine D₂R, indicating A_2AR–D₂R heterodimerization. The merged images highlighted the A_2AR–D₂R localization with respect to the cell nuclei (blue-fluorescent DAPI). Scale bar: 25 μm. (B) Representative 3D volume rendering of a sample area from (A) allowing assessment of red dot localization with respect to the cell nuclei. (C) Representative image of nuclei/red dots apparently appearing as superimposed (white dotted square in the image; corresponding magnification on the right side insert) and visualized in detail through z projection (lower right insert), thus showing a localization adjacent to the nucleus, but not inside it. Scale bar: 20 μm.