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. 2021 Apr 15;12:645723. doi: 10.3389/fphys.2021.645723

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Copyright © 2021 Stocco, Sfriso, Borile, Contran, Barbon, Romanato, Macchi, Guidolin, De Caro and Porzionato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TH and S100 immunofluorescence in representative rat and human CB samples after A_2AR–D₂R PLA. Red dots showed A_2A–D₂ receptors heterodimers; anti-TH (visualized in green) and anti-S100 (visualized in yellow) staining allowed to distinguish type I and type II cells, respectively. Cell nuclei were recognizable after DAPI staining (visualized in blue) in the merge images. Scale bar: 25 μm.