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Primers Used for Quantitative Real-Time Polymerase Chain Reaction
| Gene name | Gene symbol | Gene ID | Forward primer, 5’ to 3’ | Reverse primer, 5’ to 3’ |
|---|---|---|---|---|
| Target gene | ||||
| TGR5 | GPBAR1 | 151306 | CTCCCAGGCTATCTTCCCAG | ACAGAGAGGAAGGCAGCAG |
| Housekeeping genes | ||||
| Calnexin | CANX | 821 | AAGGTTACTTACAAAGCTCC | ACTCCTTCATTTCCTCTACC |
| Ubiquitin C | UBC | 7316 | TCGCAGCCGGGATTTGG | TCACGAAGATCTGCATTGTCAAG |
| Proteasome 20S subunit β3 | PSMB3 | 5691 | GTCCACATCATCGAGAAGGACA | GGCTCTGGGAACAGGGTTAG |
