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. 2020 Dec 16;12(1):1–24. doi: 10.1016/j.jcmgh.2020.12.010

Figure 2.

Figure 2

mNCCs give rise to neurons and glia within the gut mesentery. (A) Representative midgut segment from E14.5 Wnt1Cre;R26tdTom embryos with B-FABP and TUJ1 staining. (A’) Magnified view of the boxed area in panel A showing mNCC-derived neurons (green arrowheads), mNCC-derived glia (white arrowheads), and mNCCs (red arrowheads). (B) Relative proportions of mNCCs, NCC-derived neurons, and NCC-derived glia within the E14.5 gut mesentery (n = 1026 mNCCs from 5 embryos). (C) Schematic illustration of the generation of Phox2bFLP;NeuronCre;R26FLTG transgenic mice. (D) Genetic tracing of mNCC-derived DbhGFP+, ChatGFP+, VipGFP+, and vGatGFP+ neurons (arrowheads). Note the presence of eNCC-derived DbhGFP+, ChatGFP+, VipGFP+, and vGatGFP+ neurons within the gut. (E) Quantification of the number of mNCC-derived neurons within the gut mesentery between the midgut and hindgut of E14.5 embryos (n indicates the number of samples). (F) Representative midgut segments from P7 Phox2bFLP;DbhCre;R26FLTG and Phox2bFLP;ChatCre;R26FLTG mice. Arrowheads indicate mNCC-derived DbhGFP+ and ChatGFP+ mesenteric neurons. FRT, flippase recognition target; ME, mesentery.