Table 2.
BD FACSAria™ III cell sorter configuration
| Laser | Detector | Filter Setup | Fluorochromes | |
|---|---|---|---|---|
| Violet 407 | F | 450/40 BP | DAPI / Pacific Blue / V450 / eFluor450 / BV421 / eBFP / Hoechst33258 | |
| E | 502 LP | 510/50 BP | AmCyan / V450 / eFluor506 / BV510 / Pac Green | |
| D | 600 LP | 610/20 BP | BV605 / Pac Orange | |
| C | 630 LP | 660/20 BP | Qdot 655 | |
| B | 685 LP | 710/50 BP | Qdot 705 | |
| A | 735 LP | 780/60 BP | Qdot 800 | |
| Blue 488 | C | 488/10 BP | SSC | |
| B | 502 LP | 530/30 BP | FITC / AlexaFluor 488 / GFP / CFSE / YFP | |
| A | 655 LP | 695/40 BP | PerCP-Cy5-5 / PerCP--eFluor 710 / PI | |
| Yellow-Green 561 | E | 582/15 BP | PE / Td Tomato | |
| D | 600 LP | 610/20 BP | PE-Texas Red / PE-eFluor610 / mCherry | |
| C | 630 LP | 670/14 BP | PE-Cy5 / PI | |
| B | 685 LP | 710/50 BP | PE-Cy5-5 | |
| A | 735 LP | 780/60 BP | PE-Cy7 | |
| Red 633 | C | 660/20 BP | APC / eFluor660 / Alexa Fluor 647 | |
| B | 690 LP | 730/45 BP | Alexa Fluor 700 / APC-Cy5-5 / eFluor710 | |
| A | 755 LP | 780/60 BP | APC-Cy7 / APC - H7 / APC -Alexa750 / eFluor780 | |