REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD11b-eFluor450	eBioscience	48-0112-82
Anti-mouse CD16/CD32	BD Pharmingen	553142
Anti-mouse F4/80-AlexaFluor 488	eBioscience	53-4801-82
Anti-mouse Podoplanin-PE	BioLegend	127407
Chemicals, peptides, and recombinant proteins
Collagenase I	Thermo Fisher Scientific	17100017
Dispase	Gibco	17105-041
DNAse I	Sigma-Aldrich	11284932001
Critical commercial assays
Invitrogen™ UltraComp eBeads™ Compensation Beads	Thermo Fisher Scientific	501129040
Red Blood Cell Lysing Buffer - Hybri-MaxTM	Sigma-Aldrich	R7757
RLT lysis buffer	QIAGEN	79216
Fixable Viability Dye eFluor506	eBioscience	65-0866-14
Deposited data
RNA-sequencing data	(Bieniasz-Krzywiec et al., 2019)	GSE126722: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126722
Experimental models: cell lines
4T1 Cell line	ATCC	CRL-2539
Experimental models: organisms/strains
Mouse: WT BALB/c	Animalium Gasthuisberg, Leuven, Belgium	n/a
Software and algorithms
BD FACSDivaTM software, version 8.0	BD Biosciences	23-14523-00
FlowJo™, version 10.4.2	BD Biosciences	n/a
Other
BD FACSAria™ III Cell Sorter Details regarding sorter configuration are shown inTable 2.	BD Biosciences	23-11585-02
Dulbecco’s modified Eagle’s medium (DMEM)	Gibco	41965039
Dulbecco’s phosphate-buffered saline (PBS)	Thermo Fisher Scientific	14190144
EDTA (0.5 M), pH 8.0, RNase-free	Thermo Fisher Scientific	AM9261
Falcon Round-Bottom Polystyrene Test Tubes with Cell Strainer Snap Cap, 5 mL	Thermo Fisher Scientific	352235
Falcon 15 mL Conical Centrifuge Tubes	Thermo Fisher Scientific	14-959-53A
Falcon 50 mL Conical Centrifuge Tubes	Thermo Fisher Scientific	14-432-22
Falcon 40 μm Cell Strainer	Corning	352340
Falcon 70 μm Cell Strainer	Corning	352350
FBS (fetal bovine serum)	Biochrom GmbH	S0115
gentleMACS C Tubes	Miltenyi Biotec	130-093-237
gentleMACS Octo Dissociator with Heaters	Miltenyi Biotec	130-096-427
Gibco Penicillin-Streptomycin (10.000 U/mL)	Thermo Fisher Scientific	15140122
Petri dishes, polystyrene, 60 mm × 15 mm	Merck	P5481-500EA
RPMI-1640 Medium	Thermo Fisher Scientific	11875093
Sodium chloride solution 0.15 M	Sigma-Aldrich	7647-14-5
Sterilin 30 mm Petri Dishes	Thermo Fisher Scientific	121V
Trypsin-EDTA (0.25%)	Thermo Fisher Scientific	25200056
1,5 mL Eppendorf Safe-Lock Tubes	Eppendorf	0030 120.086
96-Well cell culture plates	Thermo Fisher Scientific	8404