| Reagent | Final concentration | Amount |
|---|---|---|
| Sodium deoxycholate (SDC) | 1% | 0.5 g |
| TCEP | 10 mM | 1 mL of 0.5 M solution (entire vial) |
| Tris | 100 mM | 5 mL of 1 M stock solution |
| ddH2O | 44 mL |
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