Method Node	Parameter	Settings
MS Scan Properties	Detector Type	Orbitrap
	Orbitrap Resolution	120,000
	Mass Range	Normal
	Use Quadrupole Isolation	True
	Scan Range (m/z)	375-1500
	RF Lens (%)	30
	AGC Target	custom
	Normalized AGC Target	250%
	Maximum Injection Time Mode	custom
	Maximum Injection Time (ms)	50
	Microscans	1
	Data Type	Profile
	Polarity	Positive
	Source Fragmentation	False
MIPS Properties	Monoisotopic Peak Determination	Peptide
Intensity Properties	Filter Type	Intensity Threshold
	Intensity Threshold	5.0e3
Charge State Properties	Include charge state(s)	2–5
	Include undetermined charge states	False
Dynamic Exclusion Properties	Exclude after n times	1
	Exclusion duration (s)	60
	Mass tolerance	ppm
	Low	10
	High	10
	Exclude isotopes	True
	Performed dependent scan on single charge state per precursor only	False
Data-Dependent MS2 Scan Properties	Isolation mode	Quadrupole
	Isolation Window (m/z)	1.4
	Isolation Offset	Off
	Activation Type	HCD
	Collision Energy Mode	Fixed
	CID Collision Energy (%)	35
	Detector Type	Ion Trap
	Ion Trap Scan rate	Rapid
	Mass Range	Normal
	Scan Range Mode	Auto
	AGC Target	Standard
	Maximum Injection Time Mode (ms)	35
	Data Type	Centroid