| Antibodies |
|
| CD45-AF647 (anti mouse) |
BioLegend |
Cat# 103124, RRID:AB_493533
|
| DAPI |
Sigma-Aldrich |
Cat# D9542-1MG |
| CD298-PE |
BioLegend |
Cat# 341704 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Lipofectamine |
Invitrogen |
Cat# 11668-019 |
| EF1a_Firefly Luciferace-T2A-mCherry (mCherry/Luc) |
Biosettia |
GlowCell 15-10 |
| 1× DPBS |
Gibco |
Cat# 14190-094 |
| Sodium deoxycholate (SDC) |
Thermo Scientific |
Cat# 89904 |
| Trizma® base (TRIS) |
Sigma |
Cat# T6791 |
| Tris(2-carboxyethyl)phosphine hydrochlorid (TCEP) |
Sigma |
Cat# 646547 |
| Ammonium bicarbonate |
Sigma |
Cat# 09830 |
| Chloroacetamide |
Sigma |
Cat# C0267 |
| Trifluoroacetic acid (TFA) |
Thermo Scientific |
Cat# 28904 |
| Porcine trypsin |
Promega |
Cat# V5113 |
| 2-Propanol |
Merck |
Cat# 109634 |
| Ammonium hydroxide solution |
Sigma |
Cat# 17093 |
| HPLC water |
Fisher Scientific |
Cat# W6-212 |
| Acetonitrile |
Thermo Scientific |
Cat# A955-212 |
| Formic acid |
Sigma |
Cat# 94318 |
| BME |
R&D Biosystems |
Cat# 3533-010-02 |
|
| Critical commercial assays |
|
| GOLD BSA removal kit |
Abcam |
Cat# ab204912 |
| Reducing agent compatible BCA assay |
Thermo Fisher |
Cat# 23250 |
| SDB-RPS cartridges |
PreOmics |
Cat# P.O.00013 |
|
| Software and algorithms |
|
| Progenesis QI |
Waters |
n/a |
| Mascot |
Matrix Science |
n/a |
| SafeQuant R package |
GitHub |
n/a |
|
| Experimental models: cell lines |
|
| MDA-MB-231 LM2 |
Massague Lab, MSKCC |
n/a |
|
| Experimental models: organisms/strains |
|
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ |
The Jackson Laboratory |
Cat# 5557 |
|
| Other |
|
| Benchtop centrifuge |
n/a |
n/a |
| Thermomixer |
Eppendorf |
Cat #EP5382000023 |
| Biruptor Pico |
Diagenode |
Cat# B01060010 |
| VialTweeter sonicator |
Hielscher |
model UIS250v |
| Vacuum concentrator |
Labconco |
Cat# 7810016 |
| Nozzle Tip, 200 Micron |
BD Biosciences |
Cat# 645895 |
| Plate reader/ nanodrop |
BMG Labtech |
SpectroStar nano |
| EASY-nLC 1200 system |
Thermo Scientific |
Cat# LC140 |
| Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
Thermo Scientific |
n/a |