Skip to main content
. 2021 Apr 17;2(2):100480. doi: 10.1016/j.xpro.2021.100480
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CD45-AF647 (anti mouse) BioLegend Cat# 103124, RRID:AB_493533
DAPI Sigma-Aldrich Cat# D9542-1MG
CD298-PE BioLegend Cat# 341704

Chemicals, peptides, and recombinant proteins

Lipofectamine Invitrogen Cat# 11668-019
EF1a_Firefly Luciferace-T2A-mCherry (mCherry/Luc) Biosettia GlowCell 15-10
1× DPBS Gibco Cat# 14190-094
Sodium deoxycholate (SDC) Thermo Scientific Cat# 89904
Trizma® base (TRIS) Sigma Cat# T6791
Tris(2-carboxyethyl)phosphine hydrochlorid (TCEP) Sigma Cat# 646547
Ammonium bicarbonate Sigma Cat# 09830
Chloroacetamide Sigma Cat# C0267
Trifluoroacetic acid (TFA) Thermo Scientific Cat# 28904
Porcine trypsin Promega Cat# V5113
2-Propanol Merck Cat# 109634
Ammonium hydroxide solution Sigma Cat# 17093
HPLC water Fisher Scientific Cat# W6-212
Acetonitrile Thermo Scientific Cat# A955-212
Formic acid Sigma Cat# 94318
BME R&D Biosystems Cat# 3533-010-02

Critical commercial assays

GOLD BSA removal kit Abcam Cat# ab204912
Reducing agent compatible BCA assay Thermo Fisher Cat# 23250
SDB-RPS cartridges PreOmics Cat# P.O.00013

Software and algorithms

Progenesis QI Waters n/a
Mascot Matrix Science n/a
SafeQuant R package GitHub n/a

Experimental models: cell lines

MDA-MB-231 LM2 Massague Lab, MSKCC n/a

Experimental models: organisms/strains

Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ The Jackson Laboratory Cat# 5557

Other

Benchtop centrifuge n/a n/a
Thermomixer Eppendorf Cat #EP5382000023
Biruptor Pico Diagenode Cat# B01060010
VialTweeter sonicator Hielscher model UIS250v
Vacuum concentrator Labconco Cat# 7810016
Nozzle Tip, 200 Micron BD Biosciences Cat# 645895
Plate reader/ nanodrop BMG Labtech SpectroStar nano
EASY-nLC 1200 system Thermo Scientific Cat# LC140
Orbitrap Fusion Lumos Tribrid Mass Spectrometer Thermo Scientific n/a