FIGURE 2.

TLB changed behaviors in aging C. elegans. (A) The number of progeny per day and the total number of progeny of C. eleganss treated with 250 μM TLB at the onset of age-synchronized late L4 or young larvae C. elegans (n = 10). (B) Age-synchronized late L4 larvae or young C. elegans were bred on NGM agar plates with or without 250 μM TLB at 20 °C for 3, 6, and 9 days, C. elegans body movement were counted with times of sinusoidal motion during 20 s (n = 20). (C) The effects of TLB on food intake in C. elegans were determined the times of the pharynx contraction for 20 sat 20°C for 3, 6, and 9 days (n = 10). p values were calculated by independent-samples t test. (D) The survival curves of eat2 (DA1116) C. elegans raised on 0 and 250 μM TLB at 20°C (n = 82–87). The lifespan was analyzed by the Kaplan-Meier test, and p values were calculated by the log-rank test. (E) Representive lipofuscin autofluorescence image of 0 and 250 μM TLB C. elegans on the 5 and 10 days of adulthood. The fluorescence of C. elegans were observed under a fluorescence microscopy. (F) The mean pixel fluorescence of transgenic C. elegans were quantified using the Image Pro Plus 6.0 software. Magnification × 100; scale bar, 100 μm (n = 20). Data were presented as mean ± SEM. ^* p < 0.05 vs. non-TLB treated controls.