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. 2021 Apr 15;12:530488. doi: 10.3389/fimmu.2021.530488

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Copyright © 2021 Liu, Du, Zhou, Zhong, Ba, Zhang, Liu and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of miR-21 on the expression of NF-κB P65 and IL-10 in HNEpC. HNEpC was transfected with miR-21 mimics (with mNC as control) and inhibitor (with iNC as control) for 24h. (A) p-P65 and P65 protein expression was determined by WB, normalized to β-actin. (B, C) Relative p-P65 and P65 protein expression were quantified by densitometry based on immunoblot images. (D, E) NF-κB and IL-10 mRNA expression were measured by qPCR. Data were obtained in three independent experiments. One-way ANOVA was used to analyze the difference between multiple groups. The asterisk indicates statistical significance, *P < 0.05; **P < 0.01; ***P < 0.001.