Figure 4.

O₃-induced expression alterations in inflammation and pathogenic infection associated APP genes. Lung and liver mRNA expression was assessed using qRT-PCR after exposure of rats to air or ozone (O₃) (1 ppm, 6 hr/day) for 1 (Day 1) or 2 (Day 2) consecutive days. Relative fold changes from air controls measured in lung and liver tissue samples are indicated in box and whisker plots, along with captured PCR amplification plots (note that Actb was similarly expressed at a given RNA concentration in both tissues); A. haptoglobin (Hp), B. ceruloplasmin (Cp), and C. hepcidin (Hamp). PCR plots are included to compare relative abundance of mRNA in liver versus lung. Box and whisker plots show all points (n=4-5/group). A significant (p ≤ 0.05) O₃ effect is indicated by an *.