Figure 9.

Early progeny of aISCs are not quiescent in homeostasis. (A) Experimental design labeling early progeny cells and aISCs with BrdU in Lgr5^eGFP-CreERT2 reporter mice not treated with DXR. Mice were injected with TAM 24 hours prior to collection to label aISCs and early progeny, and injected with BrdU 2 hours prior to collection to label cells in S-phase. (B) Representative single-color and composite images of a jejunal crypt after BrdU uptake. A BrdU⁺/tdTomato⁺ early progeny cell is outlined by the white dotted line. Scale bar = 5 μm. (C) Quantification of BrdU incorporation within the indicated populations. Data are presented as mean ± SD, n = 3 (10 crypts/mouse). ∗∗∗P < .001; ∗∗∗∗P < .0001; 1-way analysis of variance followed by Tukey’s post hoc test. ns, no significance.