Table 1.
Primer sequences for quantitative real-time PCR
| Gene | Primers |
|
|---|---|---|
| Forward | Reverse | |
| Abcg5 | TGTCCTACAGCGTCAGCAACC | GGCCACTCTCGATGTACAAGG |
| Acaca | AACGTGCAATCCGATTTGTT | GAGCAGTTCTGGGAGTTTCG |
| Acta2 | ACTACTGCCGAGCGTGAGAT | AGGTAGACAGCGAAGCCA |
| Angptl3 | ACATGTGGCTGAGATTGCTGG | CCTTTGCTCTGTGATTCCATGTAG |
| Angptl4 | GGAAAGAGGCTTCCCAAGAT | TCCCAGGACTGGTTGAAGTC |
| Angptl8 | CACTGTACGGAGACTACAAGTGC | GTGGCTCTGCTTATCAGCTCG |
| Bsep | CTGCCAAGGATGCTAATGCA | CGATGGCTACCCTTTGCTTCT |
| Cidea | CTCGGCTGTCTCAATGTCAA | CCGCATAGACCAGGAACTGT |
| Cyp27a1 | TCTGGCTACCTGCACTTCCT | CTGGATCTCTGGGCTCTTTG |
| Cyp7a1 | CAGGGAGATGCTCTGTGTTCA | AGGCATACATCCCTTCCGTGA |
| Cyp7b1 | CAGCTATGTTCTGGGCAATG | TCGGATGATGCTGGAGTATG |
| Cyp8b1 | GGACAGCCTATCCTTGGTGA | CGGAACTTCCTGAACAGCTC |
| Fasn | GCGCTCCTCGCTTGTCGTCT | TAGAGCCCAGCCTTCCATCTCCTG |
| Hmgcr | CCGGCAACAACAAGATCTGTG | ATGTACAGGATGGCGATGCA |
| Il1b | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
| Il6 | ACCACGGCCTTCCCTACTTC | CTCATTTCCACGATTTCCCAG |
| Ldlr | GCATCAGCTTGGACAAGGTGT | GGGAACAGCCACCATTGTTG |
| Ppargc1a | TGCTAGCGGTTCTCACAGAG | AGTGCTAAGACCGCTGCATT |
| Prdm16 | ACTTTGGATGGGAGCAGATG | CTCCAGGCTCGATGTCCTTA |
| Rplp0 | GGACCCGAGAAGACCTCCTT | GCACATCACTCAGAATTTCAATGG |
| Scd1 | GCGATACACTCTGGTGCTCA | CCCAGGGAAACCAGGATATT |
| Tgfb1 | TGCGCTTGCAGAGATTAAAA | CTGCCGTACAACTCCAGTGA |
| Tnfa | AGCCCACGTCGTAGCAAACCAC | TCGGGGCAGCCTTGTCCCTT |
| Ucp1 | TCAGGATTGGCCTCTACGAC | TGCATTCTGACCTTCACGAC |
Abcg5, ATP-binding cassette subfamily G member 5; Acaca, acetyl-CoA carboxylase 1; Bsep, bile salt export pump; Cidea, cell death-inducing DFFA-like effector A; Cyp27a1, sterol 27-hydroxylase; Cyp7a1, cholesterol 7 alpha-hydroxylase; Cyp7b1, oxysterol and steroid 7-alpha-hydroxylase; Cyp8b1, sterol 12-alpha-hydroxylase; Fasn, fatty acid synthase; Hmgcr, HMG-CoA reductase; Il1b, interleukin 1 beta; Il6, interleukin 6; Ldlr, low density lipoprotein receptor; Mttp, microsomal triglyceride transfer protein; Ppargc1a, peroxisome proliferative-activated receptor gamma coactivator 1 alpha; Prdm16, PR/SET domain 16; Rplp0, ribosomal protein lateral stalk subunit P0; Scd1, stearoyl-CoA desaturase 1; Tgfb1, transforming growth factor beta 1; Tnfa, tumor necrosis factor alpha.