Fig. 2.

CPT-induced nuclear translocation of endogenous cytochrome c. Immunofluorescence analysis of endogenous Cc in HeLa (A) and MEF (B) cells, upon treatment with 20 μM CPT for 6 h. Colocalization of green Cc fluorescence and blue nuclear staining is shown in the merged images in (A) and (B). Scale bars are 10 μm. (C) Activation of caspase-3 in HeLa cells upon 4, 6, 12, 24 and 48 h of CPT treatment (20 μM). Upper panel— Western-blot against caspase-3, showing the appearance of the active form of caspase-3 (cleaved caspase-3) after 12 h of CPT-induced DNA damage. Lower panel— Western-blot against GAPDH as loading control. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)