Fig. 5.
Cytochrome c competes with histones for binding to ANP32B1-251without affecting ANP32B nucleosome assembly activity. (A) Detailed view of the 1D 1H NMR spectra monitoring the Met80-ε-CH3 signal of reduced Cc (black trace) in the presence of ANP32B1-251 (red trace) and upon adding increasing concentrations of calf thymus histones (blue traces). (B) Details of 1D 1H NMR spectra of reduced Cc either free (black) or in the presence of BSA (orange) or histones (brown). (C) MNase assay by mixing relaxed plasmid with either histones (lane 4); histones and ANP32B1-251 (lane 5) or a mixture of histones, ANP32B1-251 and increasing amounts of Cc (lanes 6-8). Lane 2 and 3 correspond to the free plasmid, untreated and MNase-treated plasmid, respectively. Lane 1 indicates a DNA ladder marker with the size of each band represented on the left. DNA mobilities corresponding to one or two nucleosomes are marked on the right. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)