Fig. 7.

Cytochrome c leads to γ-H2AX dephosphorylation. Detection of γ-H2AX levels in nuclei of WT (A) or Cc^-/- KO (B) MEF cells at two recovery times (0 h and 3 h) after treatment with and removal of CPT. Control cells stands for those without CPT treatment. Localization of γ-H2AX (red fluorescence) in cell nuclei is inferred from the merged images in (A) and (B), in which red fluorescence overlaps with blue Hoechst staining. Scale bars in panels A and B are 10 μm. (C) Scatter plot showing quantification of γ-H2AX signal intensity within the cell nuclei. Black horizontal lines stand for the average values of data collected in each experiment. ****p ≤ 0.0001; n.s., non-significant (one-way ANOVA followed by a Tukey's post hoc test). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)