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. 2021 Apr 19;43:101970. doi: 10.1016/j.redox.2021.101970

Fig. 5.

Fig. 5

APE1 was required for NRF2 stability. (A, C) OE33 and FLO1 cells were treated with or without ABS (100 μM) for 20 min, followed by recovery in complete media with cycloheximide (CHX, 100 μg/mL) at the indicated time points. (E, G) OE33 (E) and FLO1(G) cells with stable knockdown of APE1 using sh-APE1 or sh-Ctrl were treated with cycloheximide (CHX, 100 μg/mL) at the indicated time points. The levels of APE1 and NRF2 were determined by western blotting. The band intensity of NRF2 was measured and normalized with the actin using the Quantity One software (BioRad Laboratories, USA) and normalized to β-actin of the same samples. (B, D, F and H) Half-life time (t1/2) of NRF2 was calculated and plotted using GraphPad Prism software, corresponding to A, C, E and G, respectively.