Fig. 2.

(A) Luciferase assay of cell lysates from U2OS cells transfected with distal Fbxo7-luc, or pTA-luc empty vector, along with a panel of six ETS family members. Luciferase values in triplicate, were background corrected with non-transfected cell lysate values, and normalised to co-transfected β-galactosidase levels, and expressed relative to empty vector (−). ∗p < 0.05 compared to the relevant empty control levels, n = 3. (B) Luciferase assay showing ELF4 activation of the empty vector, distal Fbxo7-luc and mutated distal Fbxo7-luc reporter constructs, n = 3. Below: the two ELF4 consensus sites in the FBXO7 promoter are shown in blue, and mutated base pairs in red. (C) Luciferase assays showing ELF4 activity on the Fbxo7-luc reporter in the presence of exogenous Fbxo7 or ligase-dead mutant Fbxo7-ΔFbox. (D) Luciferase assays in cells transfected with a panel of luciferase reporters, with or without Fbxo7, n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)