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. 2021 Apr 20;45(6):110. doi: 10.3892/or.2021.8061

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Vanillin downregulates NNMT expression and activity. (A and B) NNMT protein levels were determined by western blot analysis and are displayed as a histogram after treatment with vanillin (0, 2.5 and 3.5 mM) for 48 h in HT-29/NC and HT-29/shNNMT cells. (C) NNMT mRNA was determined by RT-qPCR and is displayed as a histogram after treatment with vanillin for 48 h in the HT-29 cells. (D) Relative 1-MNA level after treatment with vanillin for 48 h in the HT-29 cell lines. The untreated group of HT-29/NC cell was used as the control group which was set as 1 in all the histograms. Data are represented as means ± SD; n=3, *P<0.05, **P<0.01, Van, vanillin; NNMT, nicotinamide N-methyltransferase; 1-MNA, 1-methylnicotinamide.