FIGURE 2.

Turnover number values of (A) H₂ and (B) CO over time from the photoinduced reduction of protons by [Ru(bpy)₃]²⁺, CoPPIX, and Co-cyt b ₅₆₂ mutants at pH 6.0 under 1 atm CO₂. The experiments were carried out in 100 mM ascorbic acid, 1 mM [Ru(bpy)₃]²⁺, 200 mM potassium phosphate, 20 µM CoPPIX (except in the negative control), and 30 µM of corresponding apo cyt b ₅₆₂ (except for free CoPPIX; apo WT cyt b ₅₆₂ was used for the negative control) under white LED light irradiation. Colors represent [Ru(bpy)₃]²⁺ (negative control, orange side triangles), free CoPPIX (yellow squares), WT (red circles), M7A (purple up triangles), M7H (green down triangles), and H102A (dark blue diamonds). The TON for the negative control was calculated assuming a hypothetical catalyst content identical to the other samples (8 nmol) for direct comparison. The experiments were run on triplicate and error bars represent the standard deviation of the sample.