Figure 5.

N. annulifera venom (NaV) promotes C5a-C5aR1 axis-dependent edema. Mice (n=6/group) were pretreated with PMX205 (1 mg/kg), a C5aR1 inhibitor, or vehicle 24 and 1 hour before NaV injection. Following inhibitor administration, local reactions were induced by NaV inoculation into subcutaneous tissue from mice left hind paws and the swelling evaluated along the time with a caliper rule (A, B). In addition, tissue samples were obtained along the time, homogenized, and then submitted to LTB₄ (C), PGE₂ (D), TXB₂ (E), MPO (F) and KC (G) levels assessment by ELISA and CBA. Data are means ± SEM of six independent experiments. ***p ≤ 0.001 (two-tailed one-way ANOVA or two-way ANOVA, followed by Bonferroni post-test). ^### indicates a significant difference between animals treated with N. annulifera venom + Vehicle and N. annulifera venom + PMX205. The ^## symbol (240 minutes period) means the comparison between animals treated with the vehicle + N. annulifera venom and PMX205 + N. annulifera in which statistical differences are p ≤ 0.01.