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. 2021 Apr 15;12:652242. doi: 10.3389/fimmu.2021.652242

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Copyright © 2021 Silva de França, Villas-Boas, Cogliati, Woodruff, Reis, Lambris and Tambourgi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C5a-C5aR1 signaling drives moderate systemic reactions induced by N. annulifera venom (NaV). Mice (n=6/group) were pretreated with PMX205 (1 mg/kg), a C5aR1 inhibitor, or vehicle 24 and 1 hour before NaV injection. Following inhibitor or vehicle administration, moderate systemic reactions were induced by the injection of NaV sublethal dose, via intraperitoneal route. One hour after envenomation, blood samples were obtained by cardiac puncture to determine hematological changes by blood smear (A), and CCL2 (B) and IL-6 (C) systemic generation by ELISA and CBA. Data are means ± SEM of six independent experiments. ***p ≤ 0.001 (two-tailed t-test or two-way ANOVA, followed by Bonferroni post-test). ^### indicates a significant difference between animals treated with NaV + Vehicle and NaV + PMX205. ns = non-significant.