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. 2021 Apr 15;9:644811. doi: 10.3389/fchem.2021.644811

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Copyright © 2021 van der Zouwen and Witte.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of in situ probe formation with iminoboronate chemistry. A hydrazide ligand is reacted with a 2-FPBA-functionalized reactive group, for example a sulfonyl fluoride (41), to form an iminoboronate probe. The resulting probe is used without further purification. Probe labeling is visualized via a transimination exchange reaction with an α-amino hydrazide reporter group (42). This figure has been derived from van der Zouwen et al. (2020, Figure 1) and is used under a CC-BY 4.0 license.