FIGURE 3.

ROS accumulation are responsible for anti-NSCLC effects of GYZ in vitro. (A) ROS level was measured by DCFH-DA staining through a fluorescence microplate Reader in NSCLC cells treated with the indicated concentrations of GYZ. (B) Mitochondrial mass analyzed by ATP content measurement in A549 and H1975 cells treated with the indicated concentrations of GYZ. (C) Mitochondrial ROS accumulation was detected using MitoSOX Red in NSCLC cells treated with or without GYZ. (D) The MTT assay determined cell viability of NSCLC cells treated with GYZ (100 μM) in the absence or presence of 2 mM NAC for 48 h. (E) Colony formation assay of NSCLC cells treated with the indicated concentrations of GYZ in the absence or presence of 2 mM NAC. (F) Annexin V–FITC/PI staining was used to detect the apoptotic effects induced by GYZ (150 μM) in the absence or presence of 2 mM NAC for 48 h in NSCLC cells. Scale bars, 25 μm. All data are means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.