FIGURE 5.

Inhibition of autophagy augments the antitumor activity of GYZ in NSCLC cells. (A) A549 cells were treated with 3-MA (10 mM) or CQ (5 μM) in the absence or presence of GYZ (100 μM) for 48 h. Cell growth was evaluated by MTT assay. (B, C) A549 Cells were treated with the indicated concentrations of GYZ in the absence or presence of 3-MA (10 mM) or CQ (5 μM). Cell proliferation was detected by EdU incorporation assay. (D) A549 cells were treated with CQ (5 μM) or 3-MA (10 mM) in the absence or presence of GYZ (150 μM) for 2 weeks. Cell proliferation was performed by colony-formation assay. E, F) A549 cells were treated with 3-MA (10 mM) or CQ (5 μM) in the absence or presence of GYZ (150 μM) for 48 h. The cytotoxicity was examined by the release of LDH. All data are means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.