FIGURE 6.

Inhibition of Akt/mTOR signaling is responsible for GYZ-induced autophagy. (A) Immunoblot analysis of phosphorylation of mTOR, Akt, 4EBP1 and p70S6K expression in NSCLC cells treated with the indicated concentrations of GYZ for 24 h. Total mTOR, Akt, 4EBP1, and p70S6K expression was examined as the internal control, respectively. (B) Cells were transfected with empty vectors (pECE) or with constitutively active CA-Akt plasmids for about 36 h, and then cells were treated with GYZ (150 μM) for another 24 h. Akt phosphorylation, and LC3 turnover were detected by immunoblotting. (C,D) H1975 cells (C) and A549 cells (D) were transfected with constitutively active CA-Akt plasmids or with empty vectors (pECE) for about 36 h in the absence or presence of GYZ (A549 150 μM, H1975 100 μM) for 24 h. Scale bars, 10 μm. All data are means ± SD. ***p < 0.001.