FIGURE 4.

Subcellular tight junction protein distribution of claudin-5 under Campylobacter jejuni infection and resveratrol treatment. Resveratrol mitigates C. jejuni-induced re-distribution of claudin-5. HT-29/B6-GR/MR cells were treated with resveratrol and infected with C. jejuni from apical. 48 h post-infection monolayers were stained with antibodies against claudin-5 (red) and ZO-1 (green). Nuclei were stained blue with DAPI (bar 5 µm). Intensity plots point to signals of the TJ proteins along the white arrows in the merge pictures. The intensity signals appear highest in the TJ domain with a proper co-localization under control conditions and vary under infection and treatment conditions with the white arrows depicted over single cells.