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. 2021 Apr 21;16(1):384–394. doi: 10.1515/biol-2021-0037

Figure 4.

Figure 4

FUT4 was directly targeted by miR-320c. (a) The target relationship between miR-320c and FUT4 was predicted using starBase software. (b and c) The relationship between miR-320c and FUT4 was confirmed via dual-luciferase reporter and RIP assays. (d and e) Expression of FUT4 in C28/I2 cells transfected with miR-320 mimic or its inhibitor was assessed via qRT-PCR and Western blot assays. (f) TUG1 level in OA was analyzed by qRT-PCR. (g–j) The mRNA and protein expression of FUT4 in separated chondrocytes and IL-1β-stimulated C28/I2 cells were examined by qRT-PCR and Western blot assays, respectively. (k) Spearman’s correlation analysis was used to analyze the interrelation between miR-320c and TUG1. *P < 0.05.