Figure 5.

Immediate formation of the MYC : TRRAP cross-link and inhibition of cell proliferation upon curcumin addition. (A) HEK293T cells were incubated with increasing concentrations of curcumin, or the solvent dimethyl sulfoxide (DMSO), and protein extracts prepared after the indicated time points. Proteins were analyzed by immunoblotting using a MYC- or an α-tubulin specific antibody (n = 2). The position of the high-molecular weight MYC protein derivative (p494^{MYC : TRRAP}) is indicated. (B) Proliferation inhibition of HEK293T cells by curcumin. Cells were seeded onto 24-well cell culture plates. The next day, curcumin was added at the indicated final concentrations and cell densities measured every 6 h over a 3-day time period using an Incucyte live cell analysis system. Cells without treatment (DMSO) were used as reference. A representative experiment is shown (n = 2).