Figure 6.
Curcumin does not reduce the stabilities of the MYC and TRRAP proteins. (A) Equal numbers (2.5 × 105) of HEK293T cells were seeded onto MP24 wells and incubated with increasing concentrations of curcumin or the solvent dimethyl sulfoxide (DMSO), and protein extracts were prepared after the indicated time points. In addition, curcumin- or DMSO-treated cells were incubated in the presence of 25 µM cycloheximide (CHX) (B), or in the presence of CHX (25 µM) plus the proteasome inhibitor MG-132 (5 µM) (C). Protein analysis was performed by immunoblotting using MYC-, TRRAP-, or α-tubulin specific antibodies. The position of the high-molecular weight protein (p494) specifically reacting with the MYC antibody is indicated. A representative experiment (n = 2) is shown. Quantifications of the examined protein expression levels are depicted below each blot.