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. 2021 Apr 15;9:648331. doi: 10.3389/fcell.2021.648331

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Copyright © 2021 Wang and Na.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Organization of FBL clusters in NSN and SN oocytes. (A,B) FBL exhibits a clustered distribution pattern in fixed NSN GV oocytes. The clusters decrease significantly in fixed SN GV oocytes. Representative z section (A) and 3D projection (B) confocal images of exogenously expressed FBL–GFP and H2B-mCherry in oocytes are shown. Scale bar = 5 μm. (C) Bar graph shows relative FBL signal intensity to H2B signal intensity in fixed NSN and SN GV oocytes. N = 68. **P < 0.01. (D) The intensity distribution of FBL was measured along the arrow lines drawn on the images in (B). Green, NSN; purple, SN. (E,F) FBL distribution patterns in live NSN and SN GV oocytes. Representative z section (E) and 3D projection (F) confocal images of exogenously expressed FBL–GFP and H2B-mCherry in oocytes are shown. Scale bar = 5 μm. (G) The bar graph shows relative FBL signal intensity to H2B signal intensity in live NSN and SN GV oocytes. N = 39. **P < 0.01. (H) The intensity distribution of FBL was measured along the arrow lines drawn on the images in (F). Green, NSN; purple, SN. (I,J) FBL distribution patterns were confirmed with anti-FBL in fixed NSN and SN oocytes. Representative z section (I) and 3D projection (J) confocal images of endogenous FBL localization are shown. Scale bar = 5 μm. (K) The bar graph shows relative anti-FBL signal intensity to DNA signal intensity in fixed NSN and SN GV oocytes. N = 56. ****P < 0.0001. (L) The intensity distribution of anti-FBL was measured along the arrow lines drawn on the images in (J). Green, NSN; purple, SN.