TABLE 1.

Summary of the advantages and challenges of using zebrafish as a model organism.

Advantages	References
– Vertebrate animal—anatomically closer to human than other invertebrate models – Behaviors relatable to human – Close genome homology to human – Well-characterized genetics and transgenic models – Embryos are transparent and develop externally (outside mother)—real-time observation. – High fecundity – Short life cycle – Less complicated husbandry and maintenance	Barbazuk et al., 2000; Njiwa et al., 2004; Ali et al., 2011; Kalueff and Cachat, 2011; Avdesh et al., 2012; Gilbert et al., 2013; Howe et al., 2013; Hoo et al., 2016; D’Amora and Giordani, 2018; Rahman Khan and Sulaiman Alhewairini, 2019; Shehwana and Konu, 2019; Hughes et al., 2020; Robea et al., 2020
Challenges	References
– Less developed strains and transgenic models – Limited availability of molecular reagents and kits – Variable compound uptake for embryos – Different metabolism pathway and rate of compound uptake (since human embryos develop internally) – Neurogenesis ability—opposes degeneration process. – Functional difference of MAO	Arslan and Edmondson, 2010; Ali et al., 2011; Goldsmith and Jobin, 2012; Fierro et al., 2013; Gemberling et al., 2013; Lardelli, 2014; Stewart et al., 2014; Barbosa and Ninkovic, 2016; Saleem and Kannan, 2018; Marques et al., 2019; Godoy et al., 2020