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. 2021 Jan 29;12(1):141–157. doi: 10.1016/j.jcmgh.2021.01.016

Figure 1.

Figure 1

CD73 is expressed primarily on hepatocytes in normal mouse liver. t-distributed stochastic neighbor embedding (tSNE) and violin plots showing RNA sequencing analysis of the mouse liver from (A and B) fluorescence-activated cell sorter (FACS)-sorted and (C and D) microfluidic droplet captured cells showing the highest expression of the mouse CD73-encoding gene Nt5e in hepatocytes. Data were obtained from Tabula Muris. (E) Fresh-frozen liver sections were stained with antibodies against CD73 (green), and the cell-specific markers keratins K8/18 (hepatocyte), K19 (cholangiocyte), CD31 (endothelium), and F4/80 (Kupffer cells) in red. Bottom row: Magnified views of the boxed areas in the top row. Blue, 4′,6-diamidino-2-phenylindole (DAPI)-stained nuclei (DNA). (F) Immunofluorescence staining for CD73 (green) in frozen liver sections showing co-localization with the bile-canalicular marker zonula occludens 1 (ZO1) (red). Right: 4× magnified view of the boxed area in the merged panel. DAPI-stained nuclei. Blue, DAPI-stained nuclei (DNA). Scale bars: 20 μm. NK, natural killer; LSEC, liver sinusoidal endothelial cell.