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. 2021 Jan 29;12(1):141–157. doi: 10.1016/j.jcmgh.2021.01.016

Figure 4.

Figure 4

CD73-LKO mice develop spontaneous liver injury after 21 weeks of age in a sex-dependent manner. (A) Serum analysis of alanine aminotransferase (ALT) in 21- to 22-week-old male (blue) and female (red) WT (filled circles) and CD73-LKO mice (open circles). Males: n = 8 WT; n = 8 CD73-LKO; P = .08 (unpaired t test); females: n = 7 WT; n = 7 CD73-LKO. P = .3 (unpaired t test). (B) Representative H&E images of formalin-fixed liver sections from 22-week-old male and female WT and CD73-LKO mice. Scale bars: 200 μm. (C) Representative H&E images of formalin-fixed liver sections from 36- to 42-week-old male WT and CD73-LKO mice. Scale bars: 200 μm. (D) Quantification of blinded histologic scoring of hepatocyte degeneration (defined as swelling and ballooning) in 21- to 42-week-old mice. Scoring: 0, none; 4, mild swelling/focal ballooning; 8, severe swelling/extensive ballooning (scores were averaged for animals with ballooning + swelling). ∗P < .05; 1-way analysis of variance. Box-and-whisker plots of (E) albumin and (F) blood urea nitrogen (BUN) in male (blue) and female (red) WT (filled circles) and CD73-LKO mice (open circles). Same animals as in panel D. Males: n = 13 WT; n = 12 CD73-LKO; females: n = 14 WT; n = 18 CD73-LKO mice. ∗P < .05, ∗∗∗∗P < .0001; unpaired t test.