Skip to main content
. 2022 Mar 30;6:63. Originally published 2021 Mar 22. [Version 2] doi: 10.12688/wellcomeopenres.16536.2

Figure 3. Increasing contrast with colour and fluorescence imaging.

Figure 3.

( A) Fixed and sectioned animal tissue, stained with Oil-Red-O showing a range of structures (x4 lens, 0. 12 NA). ( B) Fixed, sectioned and stained root of a plant (Ginko) showing the different cell layers (x10 lens, 0.25 NA). ( C, C′) Fluorescence images of adjacent dried down puddles of green and red dye, respectively (x25 lens, 0.35 NA). ( D) Autofluorescence of pollen grains and ( D′) corresponding colour bright field image (x25 lens, 0.35 NA). (Scale bars = 0.20 mm in A, 0.35 mm in B, 0.35 mm in C and 0.04 mm in D.)