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. 2022 Mar 30;6:63. Originally published 2021 Mar 22. [Version 2] doi: 10.12688/wellcomeopenres.16536.2

Figure 5. Motorised XY sample navigation.

Figure 5.

( A – C) Using the motorised flexure-stage and an automated stitching algorithm to extend the field of view to cover a large sample (x10 lens 0.25 NA). ( A, A′) Example of a 5×5 image collection pattern of stage movements following a “snake by rows” grid with 30% tile overlap. ( B) Graticule scale imaged with the same ×10 objective indicating the dimensions of movement. ( C) Final processed stitched image of a fixed sectioned animal tissue histology slide stained with Oil-Red-O covering a 1.5×1.2 mm field. Dashed box = 1 image tile. (Scale bar in C = 0.2 mm.)