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. 2021 Apr 14;21(6):612. doi: 10.3892/etm.2021.10044

Table I.

Reactants of the PCR reaction.

Substance 1X (X=number of samples) 34X
Master Mix 2X 12.5 µl 425 µl
Forward Primer-Oligo 1 2.5 µl 85 µl
Reverse Primer-Oligo 2 2.5 µl 85 µl
Water, nuclease free 6 µl 204 µl
Total 23.5 µl 799 µl