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. 2021 Apr 15;21(6):631. doi: 10.3892/etm.2021.10063

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Biological functions of BAG2 in Jurkat cells. Transfection efficiency of BAG2-siRNA, detected using (A) reverse transcription-quantitative PCR and (B) western blotting. Effect of BAG2 downregulation on (C) cell proliferation was determined using MTT assays. (D) Apoptosis was determined and (E) quantified using flow cytometry. (F) The protein expression levels of Bcl-2 and Bax proteins were determined using and western blot analysis. (G) The ratio of Bcl-2/Bax was calculated. ^**P<0.01 vs. control-siRNA. BAG2, Bcl-2-associated athanogene; OD, optical density; siRNA, small interfering RNA.