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. 2021 Apr 15;21(6):631. doi: 10.3892/etm.2021.10063

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Biological functions of miR-325 in Jurkat cells. Jurkat cells were transfected with mimic control, miR-325 mimic, miR-325 mimic + control-plasmid or miR-325 mimic + BAG2-plasmid for 48 h. Subsequently, (A) cell proliferation, (B and C) cell apoptosis and (D) the protein expression levels of Bcl-2 and Bax were determined using an MTT assay, flow cytometry and western blot analysis, respectively. (E) The ratio of Bcl-2 to Bax was calculated and presented. ^**P<0.01 vs. mimic control; ^##P<0.01 vs. miR-325 mimic + control-plasmid. BAG2, Bcl-2-associated athanogene; miR, microRNA; OD, optical density.