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. 2021 Apr 29;12:251. doi: 10.1186/s13287-021-02286-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — a Adipogenic and osteogenic differentiation analysis of MenSCs and BMMSCs, staining the cells with oil red, and alizarin, respectively. Control cells were incubated using standard complete DMEM medium and stained with the same dyes after differentiation ended. The cells were cultivated in adipogenic/osteogenic medium for 21 days. Scale bar 100 μm. b Quantitative adipogenic and osteogenic differentiation analysis of MenSCs and BMMSCs (21 days), dissolving lipid droplets (stained with oil-red) in isopropanol, and calcium hydroxyapatite crystals (stained with alizarin red) in cetylpyridinium chloride solutions. Absorbance measured at 520 nm and 562 nm, respectively. Data are presented as ratio to control, mean ± SD. Horizontal bars represent p ≤ 0.05