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. Author manuscript; available in PMC: 2021 Apr 29.
Published in final edited form as: Gene. 2018 Feb 20;655:1–12. doi: 10.1016/j.gene.2018.02.048

Figure 5: Loss of the clock gene Bmal1 in Nav1.8+ sensory neurons leads to reduced pain-related protein in DRG.

Figure 5:

Expression of pain-related targets in the dorsal root ganglia (DRG) following OA surgery in bmal1f/f Nav1.8CreERT(−) & bmal1f/f Nav1.8CreERT(+) groups. Double immunofluorescence staining of substance P (red; 5A-5B), NGF (red; 5C-5D), CGRP (red; 5E-5F), TrkA (red; 5G-5H) and Nav1.7 (red; 5I-5J) and NeuN (green) in DRG of bmal1f/f Nav1.8CreERT(−) & bmal1f/f Nav1.8CreERT(+) groups mice. Co-localization of the two stains appear yellow. A scale bar was 20μm and is shown in white in color for all images. Quantitative analyses of substance P, NGF, CGRP, TrkA and Nav1.7 expression in DRGs shows lower expression in the bmal1f/f Nav1.8CreERT(+) group (Fig. 5K-4O). Values are mean±SEM.