Figure 4.

Monoclonal antibody 6D3 prevents SARS-CoV-2 infection

6D3 or isotype control antibodies (at indicated concentrations) were incubated with virus (100 plaque-forming units/well) for 1 h at room temperature before addition to Vero-E6 cells (5 × 10³ cells/well). Forty-eight hours post-infection the cells were fixed and stained for dsRNA or SARS-CoV-2 spike protein.

(A) Quantification of the percentage of infected cells per well by dsRNA staining.

(B) Representative fluorescence images of 6D3-mediated inhibition of virus infection (dsRNA).

(C) Quantification of the percentage of infected cells per well by spike staining.

(D) Representative fluorescence images of 6D3-mediated inhibition of virus infection (spike). Data were analyzed by t test (6D3 versus isotype control) with multiple testing correction (false discovery rate). Data are presented as the mean ± standard error of the mean. n = 3 technical replicates. Data are representative of three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See also Figure S1 for detailed results as a function of 6D3 concentration.