Figure 3.

FeTMPyP (2.5 µmol/L) increases respiration in isolated brain mitochondria. A: oxygen consumption rate (OCR) versus time graph. B: basal or state II respiration. C: state III respiration (ADP-induced). D: state IVo respiration (oligomycin-inhibited). E: state IIIu [carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP)-induced]. Mitochondria were isolated from wild-type (WT; n = 5 mice), and endothelial NO synthase knockout (eNOS-KO) mice (n = 5 mice) brains, treated with FeTMPyP (2.5 µmol/L), and respiratory parameters were measured using Agilent Seahorse XFe24 analyzer. Data are presented as means ± SE and analyzed by repeated-measures two-way ANOVA (data were log-transformed for some parameters). *P < 0.05, statistically significant.